3 articles published in JoVE
Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications Nicholas Bodenberger1, Dennis Kubiczek1, Frank Rosenau1 1Center for Peptide Pharmaceuticals, Faculty of Natural Science, Ulm University Different methods to manipulate three-dimensional architecture in protein-based hydrogels are evaluated here with respect to material properties. The macroporous networks are functionalized with a cell-adhesive peptide, and their feasibility in cell culture is evaluated using two different model cell lines.
Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation Christoph Wiegreffe1, Svenja Feldmann1, Simeon Gaessler1, Stefan Britsch1 1Institute of Molecular and Cellular Anatomy, Ulm University This protocol provides instructions for direct observation of radially migrating cortical neurons. In utero electroporation, organotypic slice culture, and time-lapse confocal imaging are combined to directly and dynamically study the effects of overexpression or downregulation of genes of interest in migrating neurons and to analyze their differentiation during development.
Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes Wolfgang Stein1, Carola Städele1, Peter Andras2 1Institute of Neurobiology, Ulm University, 2School of Computing Science & Institute of Neuroscience, Newcastle University Here we present the methodology for fast and high resolution fluorescent voltage-sensitive dye imaging of detailed activity of neurons in the crab stomatogastric ganglion.