University Hospital Muenster 2 articles published in JoVE Neuroscience Simultaneous Isolation of Principal Central Nervous System-Resident Cell Types from Adult Autoimmune Encephalomyelitis Mice Christina B. Schroeter*1, Antonia Henes*1, Anna Vogelsang1, Alexander M. Herrmann1, Stefanie Lichtenberg1, Derya Cengiz1, Vera Dobelmann1, Niklas Huntemann1, Christopher Nelke1, Susann Eichler2, Philipp Albrecht1, Sven G. Meuth*1, Tobias Ruck1 1Department of Neurology, Medical Faculty, Heinrich Heine University Duesseldorf, 2Department of Neurology, Institute of Translational Neurology, University Hospital Muenster To date, protocols for the simultaneous isolation of all principal central nervous system-resident cell types from the same mouse are an unmet demand. The protocol shows a procedure applicable in naïve and experimental autoimmune encephalomyelitis mice to investigate complex cellular networks during neuroinflammation and simultaneously reduce the required mice numbers. Medicine Orthotopic Kidney Auto-Transplantation in a Porcine Model Using 24 Hours Organ Preservation And Continuous Telemetry Wen-Jia Liu*1,2, Lisa Ernst*2, Benedict Doorschodt2, Jan Bednarsch1, Felix Becker3, Richi Nakatake2, Yuki Masano2, Ulf Peter Neumann1,4, Sven Arke Lang1, Peter Boor5, Isabella Lurje6, Georg Lurje1,6, René Tolba2, Zoltan Czigany1,2 1Department of Surgery and Transplantation, Faculty of Medicine, University Hospital RWTH Aachen, 2Institute for Laboratory Animal Science, Faculty of Medicine, University Hospital RWTH Aachen, 3Department of General-, Visceral-, and Transplantation Surgery, University Hospital Muenster, 4Department of Surgery, Maastricht University Medical Centre (MUMC), 5Institute of Pathology, Faculty of Medicine, University Hospital RWTH Aachen, 6Department of Surgery, Campus Charité Mitte | Campus Virchow-Klinikum, Charité-Universitätsmedizin Large animal models play an essential role in preclinical transplantation research. Due to its similarities to the clinical setup, the porcine model of orthotopic kidney auto-transplantation described in this article provides an excellent in vivo setting for the testing of organ preservation techniques and therapeutic interventions.
