3 articles published in JoVE
Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets Nane Griem-Krey1, Anders Bue Klein1, Matthias Herth1,2,3, Petrine Wellendorph1 1Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2Neurobiology Research Unit and CIMBI, Copenhagen University Hospital, 3Department of Clinical Physiology, Nuclear Medicine & PET, Copenhagen University Hospital The method of autoradiography is routinely used to study binding of radioligands to tissue sections for determination of qualitative or quantitative pharmacology.
Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm Anders Rehfeld1,2, Dorte Louise Egeberg Palme1,2, Kristian Almstrup1,2, Anders Juul1,2, Niels Erik Skakkebaek1,2 1Department of Growth and Reproduction, Copenhagen University Hospital, 2International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), University of Copenhagen Here, two medium-throughput assays for assessment of effects on Ca2+-signaling and acrosome reaction in human sperm are described. These assays can be used to quickly and easily screen large amounts of compounds for effects on Ca2+-signaling and acrosome reaction in human sperm.
Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH) Elena Ronander1,2, Dominique C. Bengtsson1,2, Louise Joergensen1,2, Anja T. R. Jensen1,2, David E. Arnot1,2,3 1Centre for Medical Parasitology, Department of International Health, Immunology & Microbiology, Faculty of Health Sciences, University of Copenhagen, 2Department of Infectious Diseases, Copenhagen University Hospital (Rigshospitalet), 3Institute of Infection and Immunology Research, School of Biology, University of Edinburgh Fluorescent in situ hybridization (FISH) to identify mRNA transcripts in individual cells allows analysis of polygenic activity such as the simultaneous transcription of more than one member of the var multigene family in Plasmodium falciparum infected erythrocytes 1. The technique is adaptable and can be used on different types of genes, cells and organisms.