4 articles published in JoVE

• Immunology and Infection

  
  Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples Maiken W. Rosenstierne¹, Christopher E. Jensen¹, Anders Fomsgaard^{1,2 1}Virus Research & Development Laboratory, Statens Serum Institut, ²Infectious Disease Research Unit, University of Southern Denmark Here, we present a protocol for the rapid virus nucleic acid extraction from the virus-inactivated whole blood. The extraction is performed directly in the blood collection tubes and requires no equipment or electricity. The method is not dependent on laboratory facilities and can be used anywhere (e.g., in field hospitals).

• Medicine

  
  Cavernous Nerve Stimulation and Recording of Intracavernous Pressure in a Rat Morten Hox¹, Travis Mann-Gow², Lars Lund^3,4, Peter Zvara^{1,4 1}Biomedical Laboratory and the Research Unit of Urology, Department of Clinical Research, University of Southern Denmark, ²Department of Surgery, University of Vermont, ³Research Unit of Urology, Department of Clinical Research, University of Southern Denmark, ⁴Department of Urology, Odense University Hospital This study describes a simplified surgical procedure and technique for performing cavernous nerve stimulation with the isolation of the nerve-electrode complex using silicone glue and intracavernous pressure measurement.

• Bioengineering

  
  Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy Maria Bloksgaard¹, Bjarne Thorsted², Jonathan R. Brewer², Jo G. R. De Mey^{1,3 1}Department of Cardiovascular and Renal Research, Institute of Molecular Medicine, University of Southern Denmark, ²Department of Biochemistry and Molecular Biology, University of Southern Denmark, ³Department of Cardiac, Thoracic and Vascular Surgery, Odense University Hospital We describe simultaneous mechanical testing and 3D-imaging of the arterial wall of isolated, live human resistance arteries, and Fiji and Ilastik image analyses for the quantification of elastin and collagen spatial organization and volume densities. We discuss the use of these data in mathematical models of arterial wall mechanics.

• Medicine

  
  Evaluating the Procedure for Performing Awake Cystometry in a Mouse Model Travis K. Mann-Gow*¹, Troy R. Larson*¹, Chrissie T. Wøien², Thomas M. Andersen², Karl-Erik Andersson^3,4, Peter Zvara^{1,2 1}Department of Surgery, University of Vermont, ²Department of Urology and Biomedical Laboratory, University of Southern Denmark, ³Wake Forest Institute for Regenerative Medicine, Wake Forest University, ⁴Institute for Clinical Medicine, Aarhus University This study describes the surgical procedures and experimental techniques for performing awake cystometry in a freely moving mouse. In addition, it provides experimental evidence to support its optimization and standardization.