3 articles published in JoVE
A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo Line L. Holm1,2,3, Milica Vukmanovic-Stejic4, Thomas Blauenfeldt1, Thomas Benfield2,3, Peter Andersen1, Arne N. Akbar4, Morten Ruhwald1 1Department of Infectious Disease Immunology, Center for Vaccine Research, Statens Serum Institut, 2Department of Infectious Diseases, Hvidovre Hospital, 3Institute of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 4Division of Infection and Immunity, University College London Here, we provide a demonstration of the suction blister cutaneous recall model. The model allows a simple access to study human in vivo adaptive immune responses, for instance in the context of vaccine development.
Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples Maiken W. Rosenstierne1, Christopher E. Jensen1, Anders Fomsgaard1,2 1Virus Research & Development Laboratory, Statens Serum Institut, 2Infectious Disease Research Unit, University of Southern Denmark Here, we present a protocol for the rapid virus nucleic acid extraction from the virus-inactivated whole blood. The extraction is performed directly in the blood collection tubes and requires no equipment or electricity. The method is not dependent on laboratory facilities and can be used anywhere (e.g., in field hospitals).
Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach Jakob Frimodt-Møller1, Godefroid Charbon1, Karen A. Krogfelt2, Anders Løbner-Olesen1 1Department of Biology, Section for Functional Genomics and Center for Bacterial Stress Response and Persistence (BASP), University of Copenhagen, 2Department of Microbiology and Infection Control, Statens Serum Institut Here, the power of a transposon-mediated random insertion of a non-coding DNA element was used to resolve its optimal chromosomal position.