3 articles published in JoVE

• Environment

  
  Cereal Crop Ear Counting in Field Conditions Using Zenithal RGB Images José A. Fernandez-Gallego^1,2,3, María Luisa Buchaillot^1,2, Adrian Gracia-Romero^1,2, Thomas Vatter^1,2, Omar Vergara Diaz^1,2, Nieves Aparicio Gutiérrez⁴, María Teresa Nieto-Taladriz⁵, Samir Kerfal⁶, Maria Dolors Serret^1,2, José Luis Araus^1,2, Shawn C. Kefauver^{1,2 1}Plant Physiology Section, Faculty of Biology, University of Barcelona, ²Agrotecnio, ³Programa de Ingeniería Electrónica, Facultad de Ingeniería, Universidad de Ibagué, ⁴Instituto Tecnológico Agrario de Castilla y León (ITACyL), ⁵Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), ⁶Syngenta Spain We present a protocol for counting durum wheat and barley ears, using natural color (RGB) digital photographs taken in natural sunlight under field conditions. With minimal adjustments for camera parameters and some environmental condition limitations, the technique provides precise and consistent results across a range of growth stages.

• Developmental Biology

  
  Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization María Jesús Sánchez-Calabuig^1,2, Francisco Alberto García-Vázquez³, Ricardo Laguna-Barraza¹, Carlos Barros-García⁴, Daniel García-Parraga⁵, Dimitrios Rizos¹, Alfonso Gutiérrez Adan¹, José Félix Pérez-Gutíerrez^{2 1}Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), ²Department of Animal Medicine and Surgery, School of Veterinary Medicine, Universidad Complutense de Madrid, ³Department of Physiology, Faculty of Veterinary Science, University of Murcia, Campus Mare Nostrum, ⁴Mundomar, Benidorm, ⁵ Here, we present protocols that have been successfully used for dolphin spermatozoa collection, cryopreservation, and heterologous IVF performance using bovine oocytes.

• Biology

  
  Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo Dorota N. Komar¹, Alfonso Mouriz¹, José A. Jarillo¹, Manuel Piñeiro^{1 1}Centro de Biotecnología y Genómica de Plantas (CBGP), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)-Universidad Politécnica de Madrid Chromatin immunoprecipitation is a powerful technique for the identification of DNA binding sites of Arabidopsis proteins in vivo. This procedure includes chromatin cross-linking and fragmentation, immunoprecipitation with selective antibodies against the protein of interest, and qPCR analysis of bound DNA. We describe a simple ChIP assay optimized for Arabidopsis plants.