2 articles published in JoVE

• Developmental Biology

  
  Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization María Jesús Sánchez-Calabuig^1,2, Francisco Alberto García-Vázquez³, Ricardo Laguna-Barraza¹, Carlos Barros-García⁴, Daniel García-Parraga⁵, Dimitrios Rizos¹, Alfonso Gutiérrez Adan¹, José Félix Pérez-Gutíerrez^{2 1}Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), ²Department of Animal Medicine and Surgery, School of Veterinary Medicine, Universidad Complutense de Madrid, ³Department of Physiology, Faculty of Veterinary Science, University of Murcia, Campus Mare Nostrum, ⁴Mundomar, Benidorm, ⁵ Here, we present protocols that have been successfully used for dolphin spermatozoa collection, cryopreservation, and heterologous IVF performance using bovine oocytes.

• Biology

  
  Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo Dorota N. Komar¹, Alfonso Mouriz¹, José A. Jarillo¹, Manuel Piñeiro^{1 1}Centro de Biotecnología y Genómica de Plantas (CBGP), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)-Universidad Politécnica de Madrid Chromatin immunoprecipitation is a powerful technique for the identification of DNA binding sites of Arabidopsis proteins in vivo. This procedure includes chromatin cross-linking and fragmentation, immunoprecipitation with selective antibodies against the protein of interest, and qPCR analysis of bound DNA. We describe a simple ChIP assay optimized for Arabidopsis plants.