Hebe Biolab 1 article published in JoVE Bioengineering Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds Nausika Betriu1, Lourdes Recha-Sancho1, Carlos E. Semino1,2 1Tissue Engineering Research Laboratory, Department of Bioengineering, IQS-School of Engineering, Ramon Llull University, 2Hebe Biolab Here, we present a protocol to obtain 3D-culture systems in self-assembling peptide scaffolds to promote the differentiation of dedifferentiated human articular chondrocytes into cartilage-like tissue.