Universidad de Valladolid 2 articles published in JoVE Chemistry Preparation of a Corannulene-functionalized Hexahelicene by Copper(I)-catalyzed Alkyne-azide Cycloaddition of Nonplanar Polyaromatic Units Celedonio M. Álvarez1, Héctor Barbero1, Sergio Ferrero1 1GIR MIOMeT, IU CINQUIMA, Área de Química Inorgánica, Universidad de Valladolid Here, we present a protocol to synthesize a complex organic compound comprised of three nonplanar polyaromatic units, assembled easily with reasonable yields. Neuroscience Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons María Calvo-Rodríguez1, Carlos Villalobos1, Lucía Nuñez2 1Instituto de Biologìa y Genética Molecular, Consejo Superior de Investigaciones Cientìficas, 2Departamento de Bioquìmica y Biologìa Molecular y Fisiologìa, Universidad de Valladolid Intracellular Ca2+ remodeling in aging may contribute to excitotoxicity and neuron damage, processes mediated by Ca2+ overload. We aimed at investigating Ca2+ remodeling in the aging brain using fluorescence and bioluminescence imaging of cytosolic and mitochondrial Ca2+ in long-term cultures of rat hippocampal neurons, a model of neuronal aging.
