Universitat Politecnica de Valencia 4 articles published in JoVE Bioengineering Low-Dose Gamma Radiation Sterilization for Decellularized Tracheal Grafts Néstor J. Martínez-Hernández1, Lara Milián-Medina2, Jorge Mas-Estellés3, José L. Monroy-Antón4, José L. López-Villalobos5, David Hervás-Marín6, Amparo Roig-Bataller1, Manuel Mata-Roig2,7,8 1Thoracic Surgery Department, Hospital Universitari de la Ribera, 2Pathology Department, Universitat de Valencia Facultat de Medicina i Odontologia, 3Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, 4Radiation Oncology, Hospital Universitari de la Ribera, 5Thoracic Surgery Department, Hospitales Universitarios Virgen del Rocío, 6Department of Applied Statistics and Operations Research and Quality, Universitat Politècnica de València, 7Networking Research Center on Respiratory Diseases (CIBERER), 8INCLIVA Biomedical Research Institute Obtaining sterilization is essential for tracheal tissue transplant. Herein, we present a sterilization protocol using low-dose gamma irradiation that is fully tolerated by organs. Chemistry Transformation of Organic Household Leftovers into a Peat Substitute Maria Consuelo Hernández-Soto1, Marisa Hernández-Latorre2, Borja Oliver-Tomas1,2, Estefanía Ponce1, Michael Renz1 1Instituto de TecnologÍa QuÍmica (UPV-CSIC), Universitat Politècnica de València-Consejo Superior de Investigaciones CientÍficas, 2Ingelia SL A protocol for hydrothermal carbonization of vegetable food waste in an autoclave is presented, with subsequent dry thermal treatment at 275 °C in a continuous flow reactor desorbing volatile organic substances. The aim is to produce a carbon material suitable as soil amendment product or substrate component. Environment Forced Flowering in Mandarin Trees under Phytotron Conditions Alfonso Garmendia1, Roberto Beltrán2, Carlos Zornoza3, Francisco J. García-Breijo2, José Reig4, María Dolores Raigón5, Hugo Merle2 1Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, 2Departamento de Ecosistemas Agroforestales, Universitat Politècnica de València, 3S.A. Explotaciones Agrícolas Serrano (SAEAS), 4Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Jardín Botánico Universitat de València, 5Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana, Universitat Politècnica de València Here, we present a protocol to force flowering in mandarin trees under phytotron conditions. Water stress, high illuminance and a simulated spring photoperiod allowed viable flowers to be obtained in a short period of time. This methodology allows researchers to have several flowering periods in 1 year. Bioengineering Sandwich-like Microenvironments to Harness Cell/Material Interactions José Ballester-Beltrán1,2, Myriam Lebourg1,3, Manuel Salmerón-Sánchez2 1Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, 2Division of Biomedical Engineering, School of Engineering, University of Glasgow, 3Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) The following protocol describes the procedure to assemble sandwich-like cultures to be used as an intermediate stage between bi-dimensional (2D) and three-dimensional (3D) cellular environments. The engineered systems can have applications in microscopy, biomechanics, biochemistry and cell biology assays.
