Healthcare Research Institute Princesa Hospital (IIS-Princesa) 1 article published in JoVE Immunology and Infection T Cells Capture Bacteria by Transinfection from Dendritic Cells Aranzazu Cruz-Adalia1, Guillermo Ramírez-Santiago1,2, Mónica Torres-Torresano1,2, Raquel Garcia-Ferreras1,2, Esteban Veiga Chacón1 1Cellular and Molecular Biology, Spanish National Research Council (CSIC), Spanish National Biotechnology Centre (CNB-CSIC), 2Hospital de Santa Cristina, Healthcare Research Institute Princesa Hospital (IIS-Princesa) Here a protocol is presented to measure bacterial capture by CD4+ T cells which occurs during antigen presentation via transinfection from pre-infected dendritic cells (DC). We show how to perform the necessary steps: isolation of primary cells, infection of DC, DC/T cell conjugate formation, and measurement of bacterial T cell transfection.
