Hospital Universitario Virgen del Rocio/Universidad de Sevilla 1 article published in JoVE Biology Experimental Approach to Examine Leptin Signaling in the Carotid Bodies and its Effects on Control of Breathing Mi-Kyung Shin1, Lenise J. Kim1, Candela Caballero-Eraso2, Vsevolod Y. Polotsky1 1Department of Medicine, Johns Hopkins University, 2Instituto de Biomedicina de Sevilla (IBiS), Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Hospital Universitario Virgen del Rocío/Universidad de Sevilla Our study focuses on the effects of leptin signaling in carotid body (CB) on the hypoxic ventilatory response (HVR). We performed 'loss of function' experiments measuring the effect of leptin on HVR after CB denervation and 'gain of function' experiments measuring HVR after overexpression of the leptin receptor in CB.
