Hospital Universitario Puerta de Hierro de Majadahonda 1 article published in JoVE Cancer Research Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay Cristina Galindo-Pumariño1, Alberto Herrera2, Alberto Muñoz3, Alfredo Carrato1, Mercedes Herrera4, Cristina Peña1 1Medical Oncology Department, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), CIBERONC, 2Department of Medical Oncology, Hospital Universitario Puerta de Hierro de Majadahonda, 3Instituto de Investigaciones Biomédicas Alberto Sols, Consejo Superior de Investigaciones Científicas - Universidad Autónoma de Madrid, CIBERONC, 4Department of Oncology & Pathology, Karolinska Institutet The aim of this method is to obtain fibroblast-derived 3D matrices as a natural scaffold for subsequent cellular assays. Fibroblasts are seeded in a pre-treated culture plate and stimulated with ascorbic acid for matrix generation. Matrices are decellularized and blocked to culture relevant cells (e.g., endothelial cells).
