Centro Nacional de Investigaciones Cardiovasculares (CNIC)

1 article published in JoVE

• Developmental Biology

  
  En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos Tamara Gonzalez-Costa^1,2, Jose Luis de la Pompa^1,2, Joaquim Grego-Bessa^{1,2 1}Intercellular Signalling in Cardiovascular Development and Disease Laboratory, Centro Nacional de Investigaciones Cardiovasculares (CNIC), ²Ciber de Enfermedades Cardiovasculares Classically, the endocardium of the mouse embryonic valve primordium has been analyzed using transversal, coronal, or sagittal sections. Our novel approach for en face, two-dimensional imaging of the endocardium at valvulogenic regions allows planar polarity and cell rearrangement analysis of the endocardium during valve development.