3 articles published in JoVE
A Neural Network-Based Identification of Developmentally Competent or Incompetent Mouse Fully-Grown Oocytes Federica Cavalera1, Mario Zanoni1, Valeria Merico1, Thi Thu Hien Bui1,2, Martina Belli1,3, Lorenzo Fassina4, Silvia Garagna1, Maurizio Zuccotti1 1Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia e Biotecnologie "Lazzaro Spallanzani", University of Pavia, 2Institute of Biotechnology, University of Helsinki, 3Department of Reproductive Medicine, University of California San Diego, 4Dipartimento di Ingegneria Industriale e dell'Informazione, University of Pavia Here, we present a protocol for non-invasive assessment of oocyte developmental competence performed during their in vitro maturation from the germinal vesicle to the metaphase II stage. This method combines time-lapse imaging with particle image velocimetry (PIV) and neural network analyses.
Preterm EEG: A Multimodal Neurophysiological Protocol Susanna Stjerna1, Juha Voipio2, Marjo Metsäranta3, Kai Kaila2,4, Sampsa Vanhatalo1 1Department of Children's Clinical Neurophysiology, Helsinki University Hospital, University of Helsinki, 2Department of Biosciences, University of Helsinki, 3Department of Pediatrics, Helsinki University Hospital, University of Helsinki, 4Neuroscience Center, University of Helsinki This video explains the background theory of the neonatal EEG activity and the sensory responses, followed by a live demonstration of their recording in neonatal intensive care unit.
Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation Dmitry A. Molotkov1, Alexey Y. Yukin1,2, Ramil A. Afzalov1,2, Leonard S. Khiroug1 1Neuroscience Center, University of Helsinki, 2Faculty of Biological and Enviromental Sciences, University of Helsinki This protocol describes a non-viral method of delivery of genetic constructs to a certain area of living rodent brain. The method consists of plasmid preparation, micropipette fabrication, neonatal rat pup surgery, microinjection of the construct, and in vivo electroporation.