3 articles published in JoVE
Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis Marjaana Rantala1, Virpi Paakkarinen1, Eva-Mari Aro1 1Molecular Plant Biology, Department of Biochemistry, University of Turku A protocol for the elucidation of plant thylakoid protein complex organization and composition with blue native polyacrylamide gel electrophoresis (BN-PAGE) and 2D-SDS-PAGE is described. The protocol is optimized for Arabidopsis thaliana, but can be used for other plant species with minor modifications.
Analysis of Epididymal Protein Synthesis and Secretion Wei Zhou1,2, Petra Sipilä3, Geoffry N. De Iuliis1,2, Matthew D. Dun2,4, Brett Nixon1,2 1Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, 2Hunter Medical Research Institute, 3Department of Physiology, Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, 4School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle Here, we report the immunofluorescence localization of dynamin to illustrate the protocols for the detection of proteins in paraffin-embedded mouse epididymal sections and those of an immortalized epididymal cell line (mECap18). We also describe the protocols for the isolation of secretory proteins from both epididymal fluid and conditioned cell media.
Changes in Mammary Gland Morphology and Breast Cancer Risk in Rats Sonia de Assis1, Anni Warri1,2, M. Idalia Cruz1, Leena Hilakivi-Clarke1 1Department of Oncology, Georgetown University, 2Institute of Biomedicine, University of Turku Medical Faculty Our protocol describes how to dissect the rat abdominal mammary gland and how to prepare mammary gland whole mounts. It also describes how to analyze mammary gland morphology using three end-points (number of terminal end buds, epithelial elongation and differentiation) and to use these results to predict mammary cancer risk in rats which were exposed to dietary modifications in utero or during prepuberty.