2 articles published in JoVE
A Magnetic Resonance Imaging Protocol for Stroke Onset Time Estimation in Permanent Cerebral Ischemia Bryony L. McGarry*1, Kimmo T. Jokivarsi*2, Michael J. Knight1, Olli H.J. Grohn2, Risto A. Kauppinen1 1School of Experimental Psychology and Clinical Research and Imaging Center Bristol, University of Bristol, 2Department of Neurobiology, A.I. Virtanen Institute, University of Eastern Finland A protocol for stroke onset time estimation in a rat model of stroke exploiting quantitative magnetic resonance imaging (qMRI) parameters is described. The procedure exploits diffusion MRI for delineation of the acute stroke lesion and quantitative T1 and T2 (qT1 and qT2) relaxation times for timing of stroke.
Flat-floored Air-lifted Platform: A New Method for Combining Behavior with Microscopy or Electrophysiology on Awake Freely Moving Rodents Mikhail Kislin1, Ekaterina Mugantseva1, Dmitry Molotkov1, Natalia Kulesskaya1, Stanislav Khirug1, Ilya Kirilkin2, Evgeny Pryazhnikov1,2, Julia Kolikova2, Dmytro Toptunov2, Mikhail Yuryev1, Rashid Giniatullin3, Vootele Voikar4, Claudio Rivera1, Heikki Rauvala1, Leonard Khiroug1 1Neuroscience Center, University of Helsinki, 2Neurotar LTD, 3A. I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, 4Laboratory Animal Center, University of Helsinki This method creates a tangible, familiar environment for the mouse to navigate and explore during microscopic imaging or single-cell electrophysiological recordings, which require firm fixation of the animal’s head.