2 articles published in JoVE
Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines Henna Myllymäki*1, Mirja Niskanen*1, Kaisa Oksanen1,2, Mika Rämet1,3,4,5 1BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, 2MedEngine Oy, 3Department of Pediatrics, Tampere University Hospital, 4Department of Children and Adolescents, Oulu University Hospital, 5PEDEGO Research Unit, Medical Research Center, University of Oulu Here we describe a protocol for the immunization of the adult zebrafish (Danio rerio) with a DNA-based vaccine and demonstrate the validation of a successful vaccination event. This method is suitable for the preclinical screening of vaccine candidates in various infection models.
Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish Hanna Luukinen1, Milka Marjut Hammarén1, Leena-Maija Vanha-aho1, Mataleena Parikka1,2 1Faculty of Medicine and Life Sciences, University of Tampere, 2Oral and Maxillofacial Unit, Tampere University Hospital Here, we present a protocol to model human tuberculosis in an adult zebrafish using its natural pathogen Mycobacterium marinum. Extracted DNA and RNA from the internal organs of infected zebrafish can be used to reveal the total mycobacterial loads in the fish and the host's immune responses with qPCR.