Oulu University Hospital 2 articles published in JoVE Developmental Biology Optimization of Renal Organoid and Organotypic Culture for Vascularization, Extended Development, and Improved Microscopy Imaging Susanna Kaisto1, Ulla Saarela1, Laura Dönges1, Irina Raykhel1, Ilya Skovorodkin*1, Seppo J. Vainio*1,2,3 1Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine, University of Oulu, 2InfoTech Oulu, University of Oulu, 3Biobank Borealis of Northern Finland, Oulu University Hospital This work describes two methods for studying organ development, an improved xenotransplantation setup on chorioallantoic membrane (CAM) from avian embryos that allows for vascularization of cultured embryonic organs and organoids and a novel fixed z-direction organ culture method with modified experimental conditions that allows for high-resolution time-lapse confocal imaging. Immunology and Infection Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines Henna Myllymäki*1, Mirja Niskanen*1, Kaisa Oksanen1,2, Mika Rämet1,3,4,5 1BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, 2MedEngine Oy, 3Department of Pediatrics, Tampere University Hospital, 4Department of Children and Adolescents, Oulu University Hospital, 5PEDEGO Research Unit, Medical Research Center, University of Oulu Here we describe a protocol for the immunization of the adult zebrafish (Danio rerio) with a DNA-based vaccine and demonstrate the validation of a successful vaccination event. This method is suitable for the preclinical screening of vaccine candidates in various infection models.
