3 articles published in JoVE
Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models Jeremie Cosette1,2,3, Rym Ben Abdelwahed2, Sabrina Donnou-Triffault1,4,5, Catherine Sautès-Fridman2, Patrice Flaud*3, Sylvain Fisson*1,4,5 1Genethon, 2Cordeliers Research Center, INSERM U872, 3Matter and Complex Systems Laboratory, Université Paris Diderot, 4INSERM U951, 5UMR_S951, Université d'Evry Val d'Essonne Bioluminescence imaging is a well-known tool for localizing tumors and metastases, but quantification of these images often requires complex calculations and particular instruments. We describe the easy-to-use luminoscore method, based on precise acquisition conditions, requiring no calculations, and enabling tumor burden and treatment response to be monitored in mouse models.
High Precision Zinc Isotopic Measurements Applied to Mouse Organs Frédéric Moynier1, Marie Le Borgne2 1Institut de Physique du Globe de Paris, Institut Universitaire de France, Université Paris Diderot, 2INSERM (Institut National de la Santé et de la Recherche Médicale), U1148: Laboratory for Vascular Translational Science, Université Paris Diderot We present the technique to measure with high precision zinc isotope ratios in mouse organs.
Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices Ulrike Pannasch*1, Jérémie Sibille*1,2, Nathalie Rouach1 1Neuroglial Interactions in Cerebral Physiopathology, Center for Interdisciplinary Research in Biology, CNRS UMR 7241, INSERM U1050, Collège de France, 2Paris Diderot University The preparation of acute brain slices from isolated hippocampi, as well as the simultaneous electrophysiological recordings of astrocytes and neurons in stratum radiatum during stimulation of schaffer collaterals is described. The pharmacological isolation of astroglial potassium and glutamate transporter currents is demonstrated.