Université de la Méditerranée View Institution's Website 1 article published in JoVE Biology Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans Barbara Squiban1, Jérôme Belougne1, Jonathan Ewbank1, Olivier Zugasti1 1Centre d’Immunologie de Marseille-Luminy, Université de la Méditerranée We describe a protocol using C. elegans and RNAi feeding libraries that allows automated measurement of multiple parameters such as fluorescence, size and opacity of individual worms in a population. We give one example of a screen to identify genes involved in anti-fungal innate immunity in C. elegans.