Claude Bernard University Lyon 1 3 articles published in JoVE Chemistry Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography Lucian Roiban1, Loïc Sorbier2, Charles Hirlimann3, Ovidiu Ersen3 1Univ Lyon, INSA-Lyon, Université Claude Bernard Lyon 1, 2IFP Energies nouvelles, 3Institut de Physique et Chimie des Matériaux de Strasbourg (IPCMS) This paper describes a protocol to achieve 3D chemical maps combining energy filtered imaging and electron tomography. The chemical distribution of two catalyst supports formed by elements that are difficult to distinguish by other imaging techniques was studied. Each application consists of mapping overlapped chemical elements - respectively spaced-ionization edges. Biology Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes Stephanie Chanon1, Christine Durand1, Aurelie Vieille-Marchiset1, Maud Robert2, Charna Dibner3, Chantal Simon1, Etienne Lefai1 1CarMeN Laboratory, INSERM U1060, INRA 1397, University of Lyon, 2Department of digestive and bariatric surgery, Obesity Integrated Center, University Hospital of Edouard Herriot, Hospices Civils de Lyon, Lyon 1 University, 3Division of Endocrinology, Diabetes, Hypertension and Nutrition, Department of Clinical Medicine, Faculty of Medicine, University of Geneva In this method, human primary muscle cells are cultured in vitro to obtain differentiated myotubes and glucose uptake rates are measured. We provide a detailed protocol to quantify rates in basal and insulin-stimulated states using radiolabeled [3H] 2-deoxy-D-Glucose. Environment Extraction and Characterization of Surfactants from Atmospheric Aerosols Barbara Nozière*1, Violaine Gérard*1, Christine Baduel2, Corinne Ferronato1 1CNRS, Université Claude Bernard Lyon 1, IRCELYON, F-69626, 2CNRS, ENS de Lyon, Université Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280 Methods are presented for the targeted extraction of surfactants present in atmospheric aerosols and the determination of their absolute concentrations and surface tension curves in water, including their Critical Micelle Concentration (CMC).
