University of Tours 2 articles published in JoVE Biochemistry Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs Mégane Bregeon1, Nicolas Guyot1, Sophie Réhault-Godbert1 1Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Université de Tours, Biologie des Oiseaux et Aviculture, 37380, Nouzilly, France This article reports a simple method to isolate the outer and inner perivitelline sublayers of chicken eggs while minimizing structural alteration and to optimize protein solubilization of each sublayer for proteomic analyses. Developmental Biology Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes Federica Franciosi1, Irene Tessaro1,2, Rozenn Dalbies-Tran3, Cecile Douet3, Fabrice Reigner4, Stefan Deleuze5, Pascal Papillier3, Ileana Miclea6, Valentina Lodde1, Alberto M. Luciano1, Ghylene Goudet3 1Department of Health, Animal Science and Food Safety, University of Milan, 2IRCCS. Istituto Ortopedico Galeazzi, 3PRC, CNRS, IFCE, Université de Tours, INRA, 4PAO, INRA, 5Clinique des Animaux de Compagnie et des Équidés, Université de Liège, 6University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania This manuscript describes an experimental approach to morphologically and biochemically characterize horse oocytes. Specifically, the present work illustrates how to collect immature and mature horse oocytes by ultrasound-guided ovum pick-up (OPU) and how to investigate chromosome segregation, spindle morphology, global histone acetylation, and mRNA expression.
