Universite Cote d'Azur 5 articles published in JoVE Biology Ultra-Fast Amplicon-Based Next-Generation Sequencing in Non-Squamous Non-Small Cell Lung Cancer Christophe Bontoux1,2,3,4,5, Virginie Lespinet-Fabre1,2,3,4, Olivier Bordone1,2,3,4, Virginie Tanga1,2,3,4, Maryline Allegra1,2,3,4, Myriam Salah1,2,3,4, Salomé Lalvée1,2,3,4, Samantha Goffinet1,2,3,4, Jonathan Benzaquen3,4,5,6, Charles-Hugo Marquette3,4,5,6, Marius Ilié1,2,3,4,5, Véronique Hofman1,2,3,4,5, Paul Hofman1,2,3,4,5 1Laboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice, Université Côte d’Azur, CHU de Nice, 2Institut Hospitalo-Universitaire (IHU), RespirERA, Université Côte d'Azur, Hôpital Pasteur, CHU de Nice, 4Team 4, Institute of Research on Cancer and Aging (IRCAN), CNRS INSERM, Centre Antoine-Lacassagne, Université Côte d'Azur, 6 The increase of molecular biomarkers to be tested for non-squamous non-small cell lung cancer (NS-NSCLC) care management has prompted the development of fast and reliable molecular detection methods. We describe a workflow for genomic alteration assessment for NS-NSCLC patients using an ultra-fast-next generation sequencing (NGS) approach. Bioengineering Conformable Wearable Electrodes: From Fabrication to Electrophysiological Assessment Marina Galliani1, Laura M. Ferrari2, Esma Ismailova1 1Centre CMP, Département BEL, Mines Saint-Etienne, 2 Two recent technologies-tattoo and textiles-have demonstrated promising results in cutaneous sensing. Here, we present the fabrication and evaluation methods of tattoo and textile electrodes for cutaneous electrophysiological sensing. These electronic interfaces made of conductive polymers outperform the existing standards in terms of comfort and sensitivity. Biology An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function Jennifer Jager1, Mélanie Gaudfrin1, Jérôme Gilleron1, Mireille Cormont1, Jean-François Tanti1 1 Presented here is a protocol to deliver oligonucleotides such as small-interfering RNA (siRNA), micro-RNA mimics (miRs), or anti-micro-RNA (anti-miR) into mature adipocytes to modulate protein and micro-RNA expression. Biochemistry Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes Souade Ikhlef*1, Nicolas-Frédéric Lipp*1, Maud Magdeleine1, Guillaume Drin1 1 Here, we describe protocols using fluorescent lipid sensors and liposomes to determine whether a protein extracts and transports phosphatidylserine or phosphatidylinositol 4-phosphate in vitro. Cancer Research Using 22C3 Anti-PD-L1 Antibody Concentrate on Biopsy and Cytology Samples from Non-small Cell Lung Cancer Patients Marius Ilié1,2,3, Mélanie Ngo-Mai1, Elodie Long-Mira1,2, Sandra Lassalle1,2, Catherine Butori1, Coraline Bence1, Marame Hamila1, Véronique Hofman1,2,3, Paul Hofman1,2,3 1Institute for Research on Cancer and Aging in Nice (Inserm U1081 and CNRS 7284), Université Côte d’Azur, 3 To expand the ability of laboratories worldwide to assess the eligibility of patients with lung cancer for treatment with pembrolizumab, in a reliable and reproducible manner, we developed an assay that uses the 22C3 antibody concentrate on a widely available immunohistochemical autostainer, for both biopsy and cytology specimens.
