University of Nice Sophia Antipolis 3 articles published in JoVE Developmental Biology Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis Tatiana Gritsaenko1, Valérie Pierrefite-Carle1, Gaëlle Creff2, Claude Vidaud3, Georges Carle1, Sabine Santucci-Darmanin1 1UMR E-4320 TIRO-MATOs CEA/DRF/BIAM, Université Nice Sophia-Antipolis, 2UMR 7272 Institut de Chimie de Nice CNRS, Université Nice Sophia-Antipolis, 3CEA, Direction de la Recherche Fondamentale (DRF), Biosciences and Biotechnologies Institute (BIAM) Uranium is known to affect bone metabolism. Here, we present a protocol aimed at investigating the effect of natural uranium exposure on the viability, the differentiation, and the function of osteoclasts, the cells in charge of bone resorption. Biology Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane Nina Milosavljevic*1, Mallorie Poët*1, Michael Monet1, Eléonore Birgy-Barelli1, Isabelle Léna1, Laurent Counillon1 1Université Nice-Sophia Antipolis, Laboratoire de Physiomédecine Moléculaire, CNRS UMR7370, and Laboratories of Excellence Ion Channel Science and Therapeutics The first part of this article shows how to select mutant cell lines expressing vesicular Na+/H+ exchangers at their plasma membrane. The second part provides protocols based on intracellular pH measurements and fast ion uptake, which are used to determine the ion selectivity and the kinetic parameters of these exchangers. Biology Biochemical Titration of Glycogen In vitro Joffrey Pelletier1, Grégory Bellot1, Jacques Pouysségur1, Nathalie M. Mazure1 1Institute for Research on Cancer and Ageing of Nice, UMR CNRS 7284 - INSERM, U1081 - UNS, Centre Antoine Lacassagne, University of Nice - Sophia Antipolis We describe here an accurate, reproducible and convenient biochemical method for titration of glycogen in vitro. This technique uses the Abcam Glycogen assay kit and is based on successive hydrolysis of glycogen to glucose and glucose titration by fluorescence.
