Biosciences and Biotechnologies Institute (BIAM) 2 articles published in JoVE Developmental Biology Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis Tatiana Gritsaenko1, Valérie Pierrefite-Carle1, Gaëlle Creff2, Claude Vidaud3, Georges Carle1, Sabine Santucci-Darmanin1 1UMR E-4320 TIRO-MATOs CEA/DRF/BIAM, Université Nice Sophia-Antipolis, 2UMR 7272 Institut de Chimie de Nice CNRS, Université Nice Sophia-Antipolis, 3CEA, Direction de la Recherche Fondamentale (DRF), Biosciences and Biotechnologies Institute (BIAM) Uranium is known to affect bone metabolism. Here, we present a protocol aimed at investigating the effect of natural uranium exposure on the viability, the differentiation, and the function of osteoclasts, the cells in charge of bone resorption. Biology An Easy Method for Plant Polysome Profiling Cécile Lecampion1,2,3, Maïna Floris1,2,3,4, Jean Raphaël Fantino5,6, Christophe Robaglia1,2,3, Christophe Laloi1,2,3 1Laboratoire de Génétique et Biophysique des Plantes, Aix-Marseille Université, 2UMR 7265 Biologie Végétale & Microbiologie Environnementales, CNRS, 3BIAM, CEA, 4Department of Biology, Biocenter, University of Copenhagen, 5Laboratoire de Chimie Bactérienne, 6CNRS, LCB UMR 7283, Aix Marseille Université This protocol describes an easy method to extract and fractionate transcripts from plant tissues on the basis of the number of bound ribosomes. It allows a global estimate of translation activity and the determination of the translational status of specific mRNAs.
