Institute Cancer De La Loire Lucien Neuwirth 1 article published in JoVE Cancer Research Evaluation of the Cell Invasion and Migration Process: A Comparison of the Video Microscope-based Scratch Wound Assay and the Boyden Chamber Assay Jean-Baptiste Guy*1,2, Sophie Espenel*1,2, Alexis Vallard1,2, Priscillia Battiston-Montagne1, Anne-Sophie Wozny1,3, Dominique Ardail1,3, Gersende Alphonse1,3, Chloé Rancoule1,2, Claire Rodriguez-Lafrasse1,3, Nicolas Magne1,2 1UMR CNRS 5822 /IN2P3, IPNL, PRISME, Laboratoire de Radiobiologie Cellulaire et Moléculaire, Faculté de Médecine Lyon-Sud, Université Lyon 1, 2Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, 3Hospices Civils de Lyon, Centre Hospitalier Lyon-Sud This study reports two different methods for the analysis of cell invasion and migration: the Boyden chamber assay and the in vitro video microscope-based wound-healing assay. The protocols for these two experiments are described, and their benefits and disadvantages are compared.
