CHU Nantes 5 articles published in JoVE Medicine Single-Anastomosis Duodeno-Ileal Bypass with Sleeve Gastrectomy Model in Mice Samuel Frey1,2, Audrey Ayer1, Thibaud Sotin1, Victoria Lorant1, Bertrand Cariou1, Claire Blanchard*1,2, Cédric Le May*1 1CHU Nantes, CNRS, INSERM, l'institut du thorax, Nantes Université, 2Chirurgie Cancérologique, Digestive et Endocrinienne, Institut des Maladies de l'Appareil Digestif, Hôtel Dieu, CHU Nantes Single-anastomosis duodeno-ileal bypass (SADI-S) is an emerging bariatric procedure with important metabolic effects. In this article, we present a reliable and reproducible model of SADI-S in mice. Cancer Research Potentiation of Anticancer Antibody Efficacy by Antineoplastic Drugs: Detection of Antibody-drug Synergism Using the Combination Index Equation Meriem Bahri*1, Julien Fleurence*1, Sébastien Faraj*1,2, Mohamed Ben Mostefa Daho*1, Sophie Fougeray*1,3, Stéphane Birklé*1,3 1Centre de Recherche en Cancérologie et Immunologie Nantes Angers (CRCINA), Institut national de la santé et de la recherche médicale (INSERM), Université d'Angers, Université de Nantes, Nantes, France, 2Service de chirurgie pédiatrique, Centre hospitalier universitaire (CHU) de Nantes, Nantes, France, 3Unité de Formation et Recherche (UFR) des Sciences Pharmaceutiques et Biologiques, Université de Nantes, Nantes, France This protocol describes how to assess synergism between an anticancer antibody and antineoplastic drugs in preclinical models by using the combination index equation of Chou and Talalay. Immunology and Infection Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood Marie-Claire Devilder1,2,3, Mélinda Moyon1,2,3, Xavier Saulquin1,2, Laetitia Gautreau-Rolland1,2 1CRCINA, Inserm, CNRS, Université d'Angers, Université de Nantes, 2LabEx IGO, "Immunotherapy, Graft, Oncology", 3CHU Nantes, France We describe a method for the production of human antibodies specific for an antigen of interest, starting from rare B cells circulating in human blood. Generation of these natural antibodies is efficient and rapid, and the antibodies obtained can discriminate between highly related antigens. Immunology and Infection In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients Séverine Bézie1,2, Claire Usal1,2, Carole Guillonneau1,2 1Centre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, 2Institut de Transplantation Urologie Néphrologie (ITUN), CHU Nantes Here, we present a protocol to induce tolerance in transplantation, and assess in vitro and in vivo the suppressive capacity of distinct cell subsets from the recipient and the immune status of the recipient toward donor or exogenous antigens. Medicine Techniques of Sleeve Gastrectomy and Modified Roux-en-Y Gastric Bypass in Mice Audrey Ayer1, Frederic Borel1, Francois Moreau1, Xavier Prieur1, Michel Neunlist2, Bertrand Cariou3, Claire Blanchard*1,4, Cedric Le May*1 1l'Institut du Thorax, INSERM, CNRS, UNIV Nantes, 2CHU Nantes, Institut des Maladies de l'Appareil Digestif, INSERM U913, 3l'Institut du Thorax, INSERM, CNRS, UNIV Nantes, CHU Nantes, 4Service de Clinique Chirurgicale Digestive et Endocrinienne, CHU de Nantes Bariatric surgery is the most efficient way to reduce body weight and the deadly metabolic complications (diabetes, obesity, and dyslipidemia) frequently associated with morbid obesity. Mouse models of bariatric surgery represent a unique asset for deciphering molecular mechanisms behind the beneficial effects of these surgeries on diabetes, hypertension, and dyslipidemia.
