INSERM U1051, Institut of Neurosciences of Montpellier 2 articles published in JoVE Neuroscience In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes Laura Dumas*1, Solène Clavreul*2, Jason Durand1, Edwin Hernandez-Garzon3, Lamiae Abdeladim4, Raphaëlle Barry-Martinet2, Alicia Caballero-Megido1, Emmanuel Beaurepaire4, Gilles Bonvento3, Jean Livet2, Karine Loulier1 1Université de Montpellier, INSERM, Institut des Neurosciences de Montpellier, 2Sorbonne Université, INSERM, CNRS, Institut de la Vision, 3Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, 4Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM, IP Paris Astrocytes tile the cerebral cortex uniformly, making the analysis of their complex morphology challenging at the cellular level. The protocol provided here uses multicolor labeling based on in utero electroporation to single out cortical astrocytes and analyze their volume and morphology with a user-friendly image analysis pipeline. Neuroscience Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo Jolanta Jagodzinska*1, Emmanuelle Sarzi*1, Mélanie Cavalier1, Marie Seveno1, Volker Baecker2, Christian Hamel1,3,4, Marie Péquignot1, Cecile Delettre1 1INSERM U1051, Institut of Neurosciences of Montpellier, 2CNRS UMS3426, BioCampus Montepellier, 3University of Montpellier, 4CHRU Montpellier, Centre of Reference for Genetic Sensory Diseases, CHU Gui de Chauliac Hospital This manuscript describes a protocol for the in vivo imaging of the mouse retina with high-resolution spectral domain optical coherence tomography (SD-OCT). It focuses on retinal ganglion cells (RGC) in the peripapillary region, with several scanning and quantifying approaches described.
