Institut Imagine 3 articles published in JoVE Neuroscience Behavioral And Physiological Analysis In A Zebrafish Model Of Epilepsy Hortense de Calbiac*1,2, Adriana Dabacan*3, Raul Muresan3, Edor Kabashi1,2, Sorana Ciura1,2 1University Paris Descartes Hospital Necker-Enfants Malades, Institut Imagine, 2Institut du Cerveau et de la Moelle épinière - ICM, Sorbonne Universités Paris, 3Transylvanian Institute of Neuroscience (TINS) Here, we present a protocol for the development and the characterization of a zebrafish model of epilepsy resulting from the transient inhibition of the DEPDC5 gene. Immunology and Infection Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α Alba Llibre*1,2, Vincent Bondet*1,2, Mathieu P. Rodero3, David Hunt4, Yanick J. Crow3,5, Darragh Duffy1,2 1Immunobiology of Dendritic Cells, Institut Pasteur, 2INSERM U1223, 3Laboratory of Neurogenetics and Neuroinflammation, INSERM UMR1163, Institut Imagine, 4MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, 5Manchester Centre for Genomic Medicine, University of Manchester Here we present a protocol to describe the development and validation of a single molecule array digital ELISA assay, which enables the ultra-sensitive detection of all IFN-α subtypes in human samples. Genetics Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions Oriana Romano1,2, Ingrid Cifola3, Valentina Poletti4, Marco Severgnini3, Clelia Peano3, Gianluca De Bellis3, Fulvio Mavilio1,4, Annarita Miccio1,2,4,5 1Center for Genome Research, Department of Life Sciences, University of Modena and Reggio Emilia, 2Laboratory of Chromatin and Gene Regulation During Development, Imagine Institute, 3Institute for Biomedical Technologies, CNR, 4Généthon, 5Sorbonne Paris Cité - Université Paris Descartes Here, we describe a protocol for genome-wide mapping of the integration sites of Moloney murine leukemia virus-based retroviral vectors in human cells.
