IGDR 1 article published in JoVE Biology Oral Bacterial Infection and Shedding in Drosophila melanogaster Jonathon A. Siva-Jothy1, Arun Prakash1, Radhakrishnan B. Vasanthakrishnan2, Katy M. Monteith1, Pedro F. Vale1,3 1Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, 2IGDR - CNRS UMR 6290, 3Centre for Immunity, Infection and Evolution, University of Edinburgh This protocol describes methods to orally expose and infect the fruit fly Drosophila melanogaster with bacterial pathogens, and to measure the number of infectious bacteria shed following gut infection. We further describe the effect of immune mutants on fly survival following oral bacterial infection.
