CNRS, LAAS 1 article published in JoVE Biology Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions Anaïs Bouissou*1, Amsha Proag*1, Marion Portes1, Vanessa Soldan2, Stéphanie Balor2, Christophe Thibault3,4, Christophe Vieu3,4, Isabelle Maridonneau-Parini1, Renaud Poincloux1 1Institut de Pharmacologie et Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, 2METi, 3CNRS, LAAS, 4Univ de Toulouse, INSA Here, we detail the experimental techniques used to evaluate the protrusion forces that podosomes apply on a compliant film, from the preparation of the film to the automated analysis of topographical images.
