2 articles published in JoVE
Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts Ulrich Jarry*1,2, Noémie Joalland*1,2, Cynthia Chauvin*1,2, Béatrice Clemenceau1,2,3, Claire Pecqueur1,2, Emmanuel Scotet1,2 1INSERM, CNRS, Université d'Angers, Université de Nantes, CRCINA, 2Immunotherapy, Graft, Oncology, LabEx IGO, 3Hopital de Nantes, Hotel Dieu Here, we present a protocol for the preparation and the stereotaxic administration of allogeneic human lymphocytes in immunodeficient mice carrying orthotopic human primary brain tumors. This study provides a proof-of-concept for both the feasibility and the antitumor efficacy of intrabrain-delivered cellular immunotherapies.
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood Marie-Claire Devilder1,2,3, Mélinda Moyon1,2,3, Xavier Saulquin1,2, Laetitia Gautreau-Rolland1,2 1CRCINA, Inserm, CNRS, Université d'Angers, Université de Nantes, 2LabEx IGO, "Immunotherapy, Graft, Oncology", 3CHU Nantes, France We describe a method for the production of human antibodies specific for an antigen of interest, starting from rare B cells circulating in human blood. Generation of these natural antibodies is efficient and rapid, and the antibodies obtained can discriminate between highly related antigens.