University Paris-Est 4 articles published in JoVE Biology Unfractionated Bulk Culture of Mouse Skeletal Muscle to Recapitulate Niche and Stem Cell Quiescence Louai Zaidan*1, Perla Geara*1, Matthew J. Borok*1, Léo Machado1, Despoina Mademtzoglou1, Philippos Mourikis1, Frederic Relaix1,2,3,4 1Univ Paris Est Creteil, INSERM, IMRB, F-94010 Creteil, France, 2Ecole nationale vétérinaire d'Alfort, IMRB, F-94700 Maisons-Alfort, France, 3EFS, IMRB, F-94010 Creteil, France, 4AP-HP, Hopital Mondor, Service d'histologie, F-94010 Creteil, France Skeletal muscle comprises multiple cell types, including resident stem cells, each with a special contribution to muscle homeostasis and regeneration. Here, the 2D culture of muscle stem cells and the muscle cell niche in an ex vivo setting that preserves many of the physiological, in vivo, and environmental characteristics are described. Biology A Capsule-Based Model for Immature Hard Tick Stages Infestation on Laboratory Mice Lourdes Mateos-Hernández1, Sabine Rakotobe1, Baptiste Defaye1,2,3, Alejandro Cabezas-Cruz1, Ladislav Šimo1 1UMR BIPAR, INRAE, Ecole Nationale Vétérinaire d'Alfort, ANSES, Université Paris-Est, 2Faculté de Pharmacie, Université de Limoges, 3UMR SPE 6134 CNRS, Université de Corte Pascal Paoli In this study, a feeding system for nymphal and larval stages of hard tick was developed using a capsule attached to laboratory mouse. The feeding capsule is made from flexible materials and remains firmly attached to the mouse for at least one week and allows comfortable monitoring of tick feeding. Biology A Versatile Model of Hard Tick Infestation on Laboratory Rabbits Consuelo Almazán1, Sarah Bonnet1, Martine Cote1, Mirko Slovák2, Yoonseong Park3, Ladislav Šimo1 1Unité Mixte de Recherche (UMR) Biologie moléculaire et immunologie parasitaires (BIPAR), Institut national de la recherche agronomique (INRA), Ecole Nationale Vétérinaire d'Alfort, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université Paris-Est, 2Institute of Zoology, Slovak Academy of Sciences, 3Department of Entomology, Kansas State University We have developed a simple and versatile system to feed hard ticks on laboratory rabbits. Our non-laborious protocol uses easily accessible materials and can be adjusted depending on the requirements of the various experimental settings. The method allows comfortable monitoring and/or sampling of ticks during the entire feeding period. Medicine Establishment of a Segmental Femoral Critical-size Defect Model in Mice Stabilized by Plate Osteosynthesis Mathieu Manassero1,2, Adeline Decambron1,2, Bui Truong Huu Thong1, Véronique Viateau1,2, Morad Bensidhoum1, Hervé Petite1 1Laboratoire de Bioingénierie et Biomécanique Ostéo-Articulaires (B2OA - UMR CNRS 7052), Université Paris Diderot, 2Ecole Nationale Vétérinaire d’Alfort, Université Paris-Est Although mouse models are invaluable tools for bone tissue engineering, models of long bone defects are sparse. This need motivated development of the present protocol which uses a locking plate with four screws and a dedicated jig to perform and stabilize a reproducible, femoral, critical-size defect with low morbidity.
