Ecole Superieure de Chimie Physique Electronique (CPE) Lyon 1 article published in JoVE Chemistry Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis Lukasz Bozycki1, Magdalena Komiazyk1, Saida Mebarek2,3,4,5,6, Rene Buchet2,3,4,5,6, Slawomir Pikula1, Agnieszka Strzelecka-Kiliszek1 1Laboratory of Lipid Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 2Université de Lyon, 3Université Lyon 1, 4 We present a protocol to compare the state of minerals in vesicles released by two human bone cell lines: hFOB 1.19 and Saos-2. Their mineralization profiles were analyzed by Alizarin Red-S (AR-S) staining, ultraviolet (UV) light visualization, transmission electron microscopy (TEM) imaging and energy dispersive X-ray microanalysis (EDX).
