Institut Cochin 3 articles published in JoVE Immunology and Infection Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue Nikaïa Smith1,2,3,4, Nassima Bekaddour2,3,4, Nicolas Leboulanger4,5, Yolande Richard*6, Jean-Philippe Herbeuval*2,3,4 1Institute of Molecular Virology, Ulm University Medical Center, 2CNRS UMR-8601, Centre Interdisciplinaire Chimie Biologie, 3Team Chemistry & Biology, Modeling & Immunology for Therapy, Université Paris Descartes, 4Université Paris Descartes, Sorbonne Paris Cité, 5Pediatric Otolaryngology Department, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Université de Paris, Institut Cochin In the present protocol, we explain how to easily process and culture tonsillar mononuclear cells from healthy humans undergoing partial surgical tonsillectomy to study innate immune responses upon activation, mimicking viral infection in mucosal tissues. Developmental Biology Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays Cyrille Ramond1,3, Antonio Bandeira2, Claire Berthault3, Pablo Pereira3, Ana Cumano3, Odile Burlen-Defranoux3 1Research Center Growth and Signaling, INSERM U845, Institut Cochin, 2Unit for Biology of Lymphocyte Populations, Immunology Department, Institut Pasteur and CIMI, Unity of Treg Biology and Therapy, University of Pierre & Marie Curie, 3Unit for Lymphopoiesis, Immunology Department, INSERM U668, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur This article describes in vivo and in vitro methodology to characterize the thymic settling progenitors by the analysis of the kinetics of generation, phenotype and numbers of their T cell progeny. Immunology and Infection Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling Laura Megrelis1,2,3, Jérôme Delon1,2,3 1Inserm U1016, Institut Cochin, 2Cnrs, UMR8104, 3Université Paris Descartes, Sorbonne Paris Cité Chemokine signaling elicits marked alterations of cellular morphology and some important redistributions of intracellular proteins. Here, a rapid and detailed protocol is provided to study these events.
