Cancer Research Center in Lyon 3 articles published in JoVE Cancer Research Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence Jessica Garcia1,2,3, David Barthelemy1,2,3, Florence Geiguer1,2, Julie Ballandier1,2,3, Kathryn W. Li4, Jean-Phillippe Aurel4, Frédérique Le Breton5, Claire Rodriguez-Lafrasse1, Brigitte Manship3, Sébastien Couraud6,7, Léa Payen1,2,3 1Laboratoire de Biochimie et Biologie Moléculaire, Groupe Hospitalier Sud, Hospices Civils de Lyon, 2Circulating Cancer (CIRCAN) Program, Hospices Civils de Lyon Cancer Institute, 3University of Lyon, Claude Bernard University, Cancer Research Center of Lyon, 4Biolidics Limited, 5Institut de Pathologie Multisites des HCL-Site Sud, Hospices Civils de Lyon, 6Acute Respiratory Disease and Thoracic Oncology Department, Lyon Sud Hospital, Hospices Civils de Lyon Cancer Institute, 7EMR-3738 Therapeutic Targeting in Oncology, Lyon Sud Medical Faculty, Lyon 1 University The characterization of circulating tumor cells (CTCs) is a popular topic in translational research. This protocol describes a semi-automatic immunofluorescence (IF) assay for PD-L1 characterization and enumeration of CTCs in non-small cell lung cancer (NSCLC) patient samples. Immunology and Infection Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents Taghreed Hirz1, Charles Dumontet1 1Immunity, Microenvironment, Virus, Cancer Research Center in Lyon Neutrophils are the most abundant type of white blood cells. The isolation of neutrophils from human blood by density gradient separation method and the differentiation of human promyelocytic (HL60) cells along the granulocytic pathway are described here; to test their role on sensitivity of lymphoma cells to anti-lymphoma agents. Biology Isolation and Culture of Mouse Primary Pancreatic Acinar Cells Johann Gout1,2,3,4,5, Roxane M. Pommier1,2,3,4,5, David F. Vincent1,2,3,4,5, Bastien Kaniewski1,2,3,4,5, Sylvie Martel1,2,3,4,5, Ulrich Valcourt*1,2,3,4,5, Laurent Bartholin*1,2,3,4,5 1INSERM U1052, Centre de Recherche en Cancérologie de Lyon, 2CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, 3Université de Lyon, 4Université Lyon 1, 5Centre Léon Bérard In this publication, we describe a rapid and convenient procedure for isolating and culturing primary pancreatic acinar cells from the murine pancreas. This method constitutes a valuable approach to study the physiology of fresh primary normal/untransformed exocrine pancreatic cells.
