Institut de Biologie de l'Ecole Normale Superieure IBENS 3 articles published in JoVE Immunology and Infection Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria Emily Morgan1, Jonah Faye Longares1, Marie-Anne Félix2, Robert J. Luallen1 1Department of Biology, San Diego State University, 2 Wild Caenorhabditis nematodes are associated with many microbes, often in the gut lumen or infecting the intestine. This protocol details a method to enrich unculturable microbes colonizing the intestine, taking advantage of the resistance of the dauer cuticle. Genetics Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis Eleftheria Saplaoura*1, Valentina Perrera*2, Vincent Colot3, Friedrich Kragler1 1Max Planck Institute of Molecular Plant Physiology, 2Department of Molecular Medicine, Medical School, University of Padua, 3 The methylated RNA immunoprecipitation assay is an antibody-based method used to enrich for methylated RNA fragments. Coupled with deep sequencing, it leads to the identification of transcripts carrying the m5C modification. Developmental Biology Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos Florence A. Giger1, Julien G. Dumortier2, Nicolas B. David1 1 Combining cell transplantation, cytoskeletal labeling and loss/gain of function approaches, this protocol describes how the migrating zebrafish prospective prechordal plate can be used to analyze the function of a candidate gene in in vivo cell migration.
