Hotel Dieu 1 article published in JoVE Immunology and Infection Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts Ulrich Jarry*1,2, Noémie Joalland*1,2, Cynthia Chauvin*1,2, Béatrice Clemenceau1,2,3, Claire Pecqueur1,2, Emmanuel Scotet1,2 1INSERM, CNRS, Université d'Angers, Université de Nantes, CRCINA, 2Immunotherapy, Graft, Oncology, LabEx IGO, 3Hopital de Nantes, Hotel Dieu Here, we present a protocol for the preparation and the stereotaxic administration of allogeneic human lymphocytes in immunodeficient mice carrying orthotopic human primary brain tumors. This study provides a proof-of-concept for both the feasibility and the antitumor efficacy of intrabrain-delivered cellular immunotherapies.
