University of Paris 13 2 articles published in JoVE Immunology and Infection Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis Thomas Labadie1, Quentin Grassin2, Christophe Batéjat2, Jean-Claude Manuguerra2, India Leclercq2,3 1Department of Infection Biology, London School of Hygiene and Tropical Medicine, 2Institut Pasteur, Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence (CIBU), 3Cellule Pasteur, Université de Paris Reported here is a protocol for the quantification of infectious viral particles using real-time monitoring of electrical impedance of infected cells. A practical application of this method is presented by quantifying influenza A virus decay under different physicochemical parameters mimicking environmental conditions. Biology The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo Anne-Sophie Perrin-Terrin1,2, Florine Jeton1,3, Aurelien Pichon1,3,4, Alain Frugière2, Jean-Paul Richalet1,3, Laurence Bodineau2, Nicolas Voituron1,3 1Sorbonne Paris Cité, Laboratory “Hypoxia & Lung” EA2363, University Paris 13, 2UPMC Univ Paris 06, INSERM, UMR_S1158 Neurophysiologie Respiratoire Expérimentale et Clinique, Sorbonne Universités, 3Laboratory of Excellence GR-Ex, 4Laboratory MOVE (EA 6314), University of Poitiers Here, we present a protocol based on c-FOS protein immunohistological detection, a classical technique used for the identification of neuronal populations involved in specific physiological responses in vivo and ex vivo.
