Université Paris Sud, UMR 967 2 articles published in JoVE Neuroscience Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics Mathieu Daynac*1,2,3,4,5, Lise Morizur*1,2,3,4, Thierry Kortulewski1,2,3,4, Laurent R. Gauthier1,2,3,4, Martial Ruat5, Marc-André Mouthon1,2,3,4, François D. Boussin1,2,3,4 1CEA DSV iRCM SCSR, Laboratoire de Radiopathologie, UMR 967, 2INSERM, UMR 967, 3Université Paris Diderot, Sorbonne Paris Cité, UMR 967, 4Université Paris Sud, UMR 967, 5CNRS, Université Paris Sud, UMR 9197, Neuroscience Paris-Saclay Institute, Molecules Circuits Department We report a Fluorescent Activated Cell Sorting (FACS)-based method to isolate neural stem cells (NSCs) and their progeny from the subventricular zone (SVZ) of the adult mouse brain. Applied to Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) transgenic mice, it allows the study of cell cycle progression by live imaging. Neuroscience Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress Olivier Etienne1,2,3,4, Amandine Bery1,2,3,4, Telma Roque1,2,3,4, Chantal Desmaze1,2,3,4, François D. Boussin1,2,3,4 1Laboratoire de Radiopathologie, CEA DSV iRCM SCSR, 2INSERM, U967, 3Université Paris Diderot, Sorbonne Paris Cité, 4Université Paris Sud, UMR 967 Administration of two analogs of thymidine, EdU and BrdU, in pregnant mice allows the analysis of cell cycle progression in neural and progenitor cells in the embryonic mouse brain. This method is useful to determine the effects of genotoxic stress, including ionizing radiation, during brain development.
