1 article published in JoVE
Primary Culture of Mouse Dopaminergic Neurons Florence Gaven1,2,3, Philippe Marin1,2,3, Sylvie Claeysen1,2,3 1CNRS, UMR-5203, Institut de Génomique Fonctionnelle, Montpellier, 2Inserm, U661, Montpellier, 3Universités de Montpellier Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various stresses.