UMR7288 CNRS, Aix-Marseille Universite 1 article published in JoVE Biology Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development Claire Chardès*1, Pauline Mélénec*1, Vincent Bertrand1, Pierre-François Lenne1 1Institut de Biologie du Développement de Marseille, UMR7288 CNRS, Aix-Marseille Université This protocol describes the setup of a light sheet microscope and its implementation for in vivo imaging of C. elegans embryos.