Inserm UMR 837 1 article published in JoVE Neuroscience Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting Francisco-Jose Fernandez-Gomez1, Fanny Jumeau1,2, Maxime Derisbourg1, Sylvie Burnouf1, Hélène Tran1, Sabiha Eddarkaoui1, Hélène Obriot1, Virginie Dutoit-Lefevre3, Vincent Deramecourt4, Valérie Mitchell2, Didier Lefranc3, Malika Hamdane1, David Blum1, Luc Buée1, Valérie Buée-Scherrer1, Nicolas Sergeant1 1Team Alzheimer & Tauopathies, Jean-Pierre Aubert Research Centre, Inserm UMR 837, 2EA 4308-Department of Reproductive Biology-Spermiology-CECOS, CHRU-Lille, 3EA2686-Laboratorie d'Immunologie, Faculté de Médecine - Pôle Recherche, 4Department of Neurology, CHRU-Lille A common protein extraction protocol using urea/thiourea/SDS buffer for human and mice brain tissue allows indentification of proteins by 2D-DIGE and their subsequent characterization by mini 2DE immunoblotting. This method enables one to obtain more reproducible and reliable results from human biopsies and experimental models.
