Institut Pasteur, CNRS UMR3559 1 article published in JoVE Immunology and Infection High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses Marianne Lucas-Hourani1, Hélène Munier-Lehmann2, Olivier Helynck2, Anastassia Komarova1, Philippe Desprès3, Frédéric Tangy1, Pierre-Olivier Vidalain1 1Unité de Génomique Virale et Vaccination, Virology Department, Institut Pasteur, CNRS UMR3569, 2Unité de Chimie et Biocatalyse, Biochemistry and Structural Biology Department, Institut Pasteur, CNRS UMR3523, 3Unité des Interactions Moléculaires Flavivirus-Hôtes, Virology Department, Institut Pasteur In vitro assays to measure virus replication have been greatly improved by the development of recombinant RNA viruses expressing luciferase or other enzymes capable of bioluminescence. Here we detail a high-throughput screening pipeline that combines such recombinant strains of measles and chikungunya viruses to isolate broad-spectrum antivirals from chemical libraries.
