Universite Toulouse 3 3 articles published in JoVE Bioengineering Automation of Bio-Atomic Force Microscope Measurements on Hundreds of C. albicans Cells Childérick Severac*1, Sergio Proa-Coronado*1,2,3, Cécile Formosa-Dague4, Adrian Martinez-Rivas3,5, Etienne Dague2 1ITAV-CNRS, Université de Toulouse, CNRS, Toulouse, France, 2LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France, 3ENCB, Instituto Politécnico Nacional (IPN), Mexico City, Mexico, 4TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France, 5CIC, Instituto Politécnico Nacional (IPN), Mexico City, Mexico This protocol aims to automate AFM measurements on hundreds of microbial cells. First, microbes are immobilized into PDMS stamp microstructures and then force spectroscopy measurements are performed automatically on hundreds of immobilized cells. Engineering Twin-Screw Extrusion Process to Produce Renewable Fiberboards Philippe Evon1, Laurent Labonne1, Saif Ullah Khan1,2,3, Pierre Ouagne3, Pierre-Yves Pontalier1, Antoine Rouilly1 1Laboratoire de Chimie Agro-industrielle (LCA), Université de Toulouse, INRAE, INPT, 2Balochistan University of Information Technology, Engineering and Management Sciences, 3Laboratoire Génie de Production (LGP), Université de Toulouse, INPT A versatile twin-screw extrusion process to provide an efficient thermo-mechano-chemical pretreatment on lignocellulosic biomass was developed, which leads to an increased average fiber aspect ratio. A natural binder can also be added continuously after fiber refining, leading to bio-based fiberboards with improved mechanical properties after hot pressing of the obtained extruded material. Immunology and Infection Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors Marion Szelechowski1, Corinne Bergeron2, Daniel Gonzalez-Dunia1, Bernard Klonjkowski2 1INSERM UMR 1043, CNRS UMR 5282, Université Toulouse 3, 2UMR Viroligie, INRA ENVA ANSES These past 15 years, canine adenovirus type 2 (CAV2)-derived vectors have proven their efficiency to transduce cells in vitro and in vivo and are widely used for vaccination and gene therapy. Here, we describe a procedure to construct, produce and purify CAV2 vectors, giving rise to high-titer viral suspensions.
