Universite Claude Bernard Lyon 2 articles published in JoVE Engineering Induction of Microstreaming by Nonspherical Bubble Oscillations in an Acoustic Levitation System Claude Inserra1, Gabriel Regnault2, Sarah Cleve2, Cyril Mauger2, Philippe Blanc-Benon2 1Univ Lyon, Université Claude Bernard Lyon 1, Centre Léon Bérard, INSERM, UMR 1032, LabTAU, France, 2Univ Lyon, Ecole Centrale de Lyon, INSA de Lyon, CNRS, LMFA UMR 5509, France A fast and reliable technique is proposed to control the shape oscillations of a single, trapped acoustic bubble that is based on coalescence technique between two bubbles. The steady-state, symmetry-controlled bubble shape oscillations allow analysis of the fluid flow generated in the vicinity of the bubble interface. Biology A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome Marie-Christine Mariol1,2, Ludivine Walter1,2, Stéphanie Bellemin1,2, Kathrin Gieseler1,2 1Université Claude Bernard Lyon, 2Centre de Génétique et de Physiologie Moléculaires et Cellulaires, CNRS UMR 5534 This protocol describes a rapid and low material consuming procedure for the integration of transgenic extrachromosomal arrays into the Caenorhabditis elegans genome using ultra violet (UV) irradiation. Furthermore, this protocol is particularly well suited for transgenic lines that transmit extrachromosomal arrays at a high rate.
