2 articles published in JoVE
Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules Stephanie Hutin1, Bart Van Laer1, Christoph Mueller-Dieckmann1, Gordon Leonard1, Didier Nurizzo1, Matthew W. Bowler2 1Structural Biology Group, European Synchrotron Radiation Facility, 2Grenoble Outstation, European Molecular Biology Laboratory Here, we describe how to use the automated screening and data collection options available at some synchrotron beamlines. Scientists send cryocooled samples to the synchrotron, and the diffraction properties are screened, the data sets are collected and processed and, where possible, a structure solution is carried out—all without human intervention.
Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect Stephanie Hutin*1, Gianluca Santoni*1, Ulrich Zander2, Nicolas Foos1, Sylvain Aumonier1, Guillaume Gotthard1, Antoine Royant1,3, Christoph Mueller-Dieckmann1, Gordon Leonard1 1European Synchrotron Radiation Facility, Structural Biology Group, 2European Molecular Biology Laboratory, 3Univ. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale) We present the use of the MeshAndCollect protocol to obtain a complete diffraction data set, for use in subsequent structure determination, composed of partial diffraction data sets collected from many small crystals of the fluorescent protein Cerulean.