1 article published in JoVE
Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns) Pierrette Courtois1,2, Patricia Nabos1,2, Romaric Nzoumbou-Boko1,2, Christine E. Reix3, Frédéric-Antoine Dauchy1,2,4, Sylvie Daulouede1,2, Frédéric Bringaud3, Derrick R. Robinson3, Philippe Vincendeau1,2,4 1Univ. Bordeaux UMR INTERTRYP 177 IRD CIRAD, 2IRD UMR 177 INTERTRYP CIRAD, 3CNRS, UMR 5234, Microbiologie Fondamentale et Pathogénicité, Univ. Bordeaux, 4Centre Hospitalier Université Bordeaux This method of trypanosome separation from blood depends on their surface charge being less negative than mammalian blood cells. Infected blood is placed and treated on an anion-exchanger column. This method, the most fitting diagnostic for African trypanosomiasis, provides purified parasites for immunological, biological, biochemical, pharmaceutical and molecular biology investigations.