3 articles published in JoVE
Double Direct Injection of Blood into the Cisterna Magna as a Model of Subarachnoid Hemorrhage Martin Pedard1, Mohamad El Amki1, Antoine Lefevre-Scelles1,2, Vincent Compère1,2, Hélène Castel1 1University of Rouen Normandy, INSERM U1239, DC2N, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France, 2Department of Anesthesiology and Critical Care, Rouen University Hospital, Rouen, France We described in this protocol a standardized subarachnoid hemorrhage (SAH) mouse model by a double injection of autologous whole-blood into the cisterna magna. The high degree of standardization of the double-injection procedure represents a middle-to-acute model of SAH with relative safety regarding mortality.
A Protocol to Set Up Needle-Free Connector with Positive Displacement on Central Venous Catheter in Intensive Care Unit Thomas Clavier1,2, Sébastien Franchina1, Antoine Lefevre-Scelles1, Philippe Gouin1, Emmanuel Besnier1,2, Benoit Veber1 1Department of Anesthesiology and Critical Care, Rouen University Hospital, 2Normandie Univ, UNIROUEN, INSERM U1096 We present a protocol to show the installation of a needle-free connector with positive displacement on a central venous catheter.
Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury Nicolas Guerout1,2, Alexandre Paviot1,3, Nicolas Bon-Mardion1,3, Axel Honoré1, Rais OBongo1,4, Célia Duclos1, Jean-Paul Marie1,3 1UPRES EA3830, Institute for Research and Innovation in Biomedicine, University of Rouen, 2Neuroscience, Karolinska Institutet, 3Otorhinolaryngology, Head and Neck Surgery Department, Rouen University Hospital, 4Otorhinolaryngology, Head and Neck Surgery Department, Amiens University Hospital Olfactory ensheathing cells (OECs) are neural crest cells which allow growth of the primary olfactory neurons. This specific property can be used for cellular transplantation. We present here a model of cellular transplantation based on the use of OECs in a laryngeal nerve injury model.