Vect-Horus S.A.S. 1 article published in JoVE Medicine Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport Yves Molino1, Françoise Jabès1, Emmanuelle Lacassagne2, Nicolas Gaudin2, Michel Khrestchatisky2 1VECT-HORUS SAS, 2Aix-Marseille Université, CNRS, NICN UMR 7259 The aim of the present study was to validate the reproducibility of an in vitro BBB model involving a rat syngeneic co-culture of endothelial cells and astrocytes. The endothelial cell monolayer presented high TEER and low LY permeability. Expression of specific TJ proteins, functional responses to inflammation and functionality of transporters and receptors were assessed.
